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Developmental Studies Hybridoma Bank
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Proteintech
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Developmental Studies Hybridoma Bank
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Becton Dickinson
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TXNL4B Antibody raised in Rabbit validated in WB,IF in Human, Mouse, Rat.
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Boster Bio Anti-Thioredoxin-like protein 4B TXNL4B Antibody catalog # A13142. Tested in WB,IF applications. This antibody reacts with Human,Mouse,Rat.
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Rabbit polyclonal antibody to DLP1 (Phospho-Ser616). Isotype Note: IgG Host Note: Rabbit Conjugation Note: Unconjugated Reactivity Note: Human, Mouse, Rat Application Note: WB, IHC-P, P-ELISA
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Image Search Results
Journal: Translational oncology
Article Title: SIRT1 Regulates the Chemoresistance and Invasiveness of Ovarian Carcinoma Cells.
doi: 10.1016/j.tranon.2017.05.005
Figure Lengend Snippet: Figure 6. A: Glutathione (GSH) levels in OvCa cells. GSH is one of the major antioxidants. Among OvCa cell lines, intracellular GSH level was significantly decreased by SIRT1-knockdown (si/shSIRT1) compared with control (si/shCon) in TOV21G cells. B and C: Western blotting showed the expression of CD44v and xCT, involved in the synthesis of GSH, and other antioxidative enzymes such as heme oxygenase-1 (HO-1) and thioredoxin (TRDX) in OvCa cell lines. Knockdown of SIRT1 by siRNA or shRNA (B) and EX527 (a SIRT1 inhibitor) (C) decreased expression of these antioxidative proteins. In contrast, overexpression of SIRT1 by SIRT1 cDNA increased expression of antioxidative proteins in ES2, and these effects of SIRT1 were canceled out by EX527 (C). Significance: * P b .05.
Article Snippet: After blocking for 1 hour with 5% skim milk, membranes were incubated with primary antibodies against SIRT1 (rabbit polyclonal; Cell Signaling, Danvers, MA, USA), HO-1 (rabbit polyclonal; Cell Signaling, Danvers, MA, USA), xCT (rabbit polyclonal; Abcam, USA), CD44v9 (rat monoclonal; Cosmo Bio, Tokyo, Japan),
Techniques: Knockdown, Control, Western Blot, Expressing, shRNA, Over Expression